Let-7-P2b1a

MirGeneDB ID

Gmo-Let-7-P2b1a

Family name

LET-7 (all species)

Species

Cod (Gadus morhua)

MiRBase ID

gmo-let-7f

Paralogues

Gmo-Let-7-P1b1 Gmo-Let-7-P1b2 Gmo-Let-7-P1c2 Gmo-Let-7-P1d1 Gmo-Let-7-P1d2 Gmo-Let-7-P2a1a Gmo-Let-7-P2a2a Gmo-Let-7-P2a3a Gmo-Let-7-P2a3b Gmo-Let-7-P2a4a Gmo-Let-7-P2a4b Gmo-Let-7-P2b2a Gmo-Let-7-P2b3a Gmo-Let-7-P2b3b Gmo-Let-7-P2b4a Gmo-Let-7-P2b4b Gmo-Let-7-P2c2a Gmo-Let-7-P2c2b Gmo-Let-7-P2c3

Orthologues

Aae-Let-7 Aca-Let-7-P2b1 Aga-Let-7 Agr-Let-7 Ami-Let-7-P2b1 Bge-Let-7 Bko-Let-7 Bpl-Let-7 Bta-Let-7-P2b1 Cfa-Let-7-P2b1 Cja-Let-7-P2b1 Cli-Let-7-P2b1 Cpi-Let-7-P2b1 Cpo-Let-7-P2b1 Cte-Let-7 Dan-Let-7 Dlo-Let-7 Dma-Let-7 Dme-Let-7 Dmo-Let-7 Dno-Let-7-P2b1 Dpu-Let-7 Dre-Let-7-P2b1a Dsi-Let-7 Dya-Let-7 Eba-Let-7 Eca-Let-7-P2b1 Egr-Let-7 Esc-Let-7 Ete-Let-7-P2b1 Gga-Let-7-P2b1 Gja-Let-7-P2b1 Gpa-Let-7 Gsa-Let-7 Gsp-Let-7 Hme-Let-7 Hmi-Let-7 Hru-Let-7 Hsa-Let-7-P2b1 Isc-Let-7 Laf-Let-7-P2b1 Lan-Let-7 Lch-Let-7-P2b1 Lgi-Let-7 Lhy-Let-7 Llo-Let-7 Loc-Let-7-P2b1 Mal-Let-7-P2b1a Mdo-Let-7-P2b1 Mgi-Let-7 Mml-Let-7-P2b1 Mmr-Let-7-P2b1 Mmu-Let-7-P2b1 Mom-Let-7 Mun-Let-7-P2b1 Neu-Let-7-P2b1 Npo-Let-7 Oan-Let-7-P2b1 Obi-Let-7 Ocu-Let-7-P2b1 Ofu-Let-7 Ovu-Let-7 Pab-Let-7-P2b1 Pau-Let-7 Pbv-Let-7-P2b1 Pca-Let-7 Pcr-Let-7 Pdu-Let-7 Pfl-Let-7 Ple-Let-7 Pmi-Let-7 Pve-Let-7 Rno-Let-7-P2b1 Rph-Let-7 Sha-Let-7-P2b1 Sko-Let-7 Sma-Let-7 Sne-Let-7 Snu-Let-7 Spt-Let-7-P2b1 Spu-Let-7 Sro-Let-7 Tca-Let-7 Tgu-Let-7-P2b1 Tur-Let-7 War-Let-7 Xbo-Let-7

Node of Origin (locus)

Clupeocephala

Node of Origin (family)

Bilateria

Genome context
(Gadus_morhua.gadMor1.dna.toplevel)

GeneScaffold_1801: 26456-26532 [-] UCSC Ensembl

Clustered miRNAs
(< 50kb from Let-7-P2b1a)

Let-7-P2b1a GeneScaffold_1801: 26456-26532 [-] UCSC Ensembl
Let-7-P2a1a GeneScaffold_1801: 26965-27035 [-] UCSC Ensembl

Seed

GAGGUAG

Precursor
(pre-Mir +30nt flank)

GCCCAGACGUCUUGAUUCGCUCAUUCAGUGUGAGGUAGUAGAUUGUAUAGUUUAAGGGUAGUGAUUUUACCCGUUUCAGAAGAUAACUAAACAGUCUACUGCCUUCCUUGAGGAGUGAUAUUGCUUCAGGUCUACUA

Get precursor sequence

Structure

        10           20        30        40        50        60        
GCCCAGACGUCUUGAUU---|    A     UGU               A     UAAGGGUAGUGAUUU 
                    CGCUC UUCAG   GAGGUAGUAGAUUGU UAGUU               U
                    GUGAG GAGUU   UUCCGUCAUCUGACA AUCAA               A
AUCAUCUGGACUUCGUUAUA^    -     CC-               A     UAGAAGACUUUGCCC 
     130       120        110        100        90        80        70

Deep sequencing

Comment

Although there is a templated T at the 3' end of the 3p arm, the post-transcriptional addition of a terminal 3' U is supported by the conservation of Let7-P2 processing and the sequenced 3' DcRNA reads.

3' NTU

Yes

Motifs

CNNC at 3p(+17)

Tissue expression

Br	Gi	He	In	Li	Sk	Sp	Wh

Mature sequence

Gmo-Let-7-P2b1a_5p

mirBase accession

MIMAT0044154

Sequence

0- UGAGGUAGUAGAUUGUAUAGUU -22

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Star sequence

Gmo-Let-7-P2b1a_3p*

mirBase accession

MIMAT0044155

Sequence

56- CUAAACAGUCUACUGCCUUCC -77

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